Cell harvesting is considered one of the main obstacles of using microcarriers in 3D cell culturing processes. With Fujifilm’s new injectable microcarrier cell harvesting can be avoided. Cell-loaded microcarriers have the unique advantage of being a cell delivery system to damaged or degenerated tissue for repair and restoration of function, and they enhance cell retention.
Leveraging on Fujifilm’s human collagen I-based CellnestTM biomaterial, a revolutionary new injectable microsphere has been developed. The microspheres can be used for cell delivery to patients after cell expansion, without the additional need of cell harvesting. The macroporous microspheres have a size range of 100-400 µm, which make them suitable for injection. Their macroporous structure provide an artificial microenvironment allowing cell-cell and cell-matrix interaction in all three dimension, together with a high nutrient and gas exchange rate.
Unique features of Fujifilm macroporous microspheres are, that:
- Microspheres act as microcarrier for cell culturing AND as injectable cell delivery vehicle.
- Cell harvesting, which is considered one of the main obstacles of using microcarriers, will not be necessary after cell expansion. Elimination of the harvesting step will avoid multiple passaging times of cells and will improve cell viability.
- Injection of cells adhered to the biomaterial enhances cell survival after injection in damaged hostile environment.
- The macroporous microspheres are biocompatible and fully biodegradable.
- There is an increased surface area and accessible volume due to the macroporosity. The higher surface volume allows cellular colonization at the inner surface, resulting in higher cell yields.
- The microspheres are suitable for upscaling dynamic expansion processes of cells in spinner flasks and bioreactors.
- The synthetic, animal product-free material has a very consistent and reproducible high quality.
- The macroporous microspheres can be supplied in both research and GMP quality.
In summary, Fujifilm's macro-porous microspheres are suitable
- As minimally invasive cell delivery vehicle to patients, without the need for cell harvesting
- As microcarriers to grow cells onto
- For anchorage-dependent cell production
- In dynamic expansion systems like spinner flasks and bioreactors