To achieve the optimal 3D matrix-cell conformation, recombinant peptide can be processed into other formulations than microspheres and hydrogels, such as porous scaffolds. These sponges or sheets can have directional or non-directional pores. With respect to optimisation for different cell types, pore sizes are tunable between 20-400 μm. As with the microspheres, these sponges can be used for (3D) cell culturing purposes in regenerative medicine or drug discovery purposes.
SEM pictures of recombinant collagen-like 1 peptide hRCP-C1 scaffolds
Panel A, B show a freeze dried sponge with directional pores in top view (A) and in vertical cross section view (B) [ref 1]. Panel C shows collagen-like 1 peptide hRCP-C1 sheet with interconnected pores in vertical cross section view [ref 2].
 Pawelec, KM, Confalonieri, D, Ehlicke, F, van Boxtel, HA, Walles, H, Kluijtmans, SGJM. 2017. Osteogenesis and mineralization of mesenchymal stem cells in collagen type I based recombinant peptide scaffolds. J Biomed Mater Res Part A 2017: 105A: 1856– 1866.
 Parvizi, M, Petersen, AH, van Spreuwel‐Goossens, CAFM, Kluijtmans, SGJM, Harmsen, MC. 2018. Perivascular scaffolds loaded with adipose tissue‐derived stromal cells attenuate development and progression of abdominal aortic aneurysm in rats. J Biomed Mater Res Part A 2018: 106A: 2494– 2506.
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