Scaffold technology

To achieve the optimal 3D matrix-cell conformation, recombinant peptide can be processed into other formulations than microspheres and hydrogels, such as porous scaffolds. These sponges or sheets can have  directional or non-directional pores. With respect to optimisation for different cell types, pore sizes are tunable between 20-400 μm. As with the microspheres, these sponges can be used for (3D) cell culturing purposes in regenerative medicine or drug discovery purposes.

Scaffolds SEM pictures ABC

SEM pictures of recombinant collagen-like 1 peptide hRCP-C1 scaffolds
Panel A, B show a freeze dried sponge with directional pores in top view (A) and in vertical cross section view (B) [ref 1]. Panel C shows collagen-like 1 peptide hRCP-C1 sheet with interconnected pores in vertical cross section view [ref 2].

References:
[1] Pawelec, KM, Confalonieri, D, Ehlicke, F, van Boxtel, HA, Walles, H, Kluijtmans, SGJM. 2017. Osteogenesis and mineralization of mesenchymal stem cells in collagen type I based recombinant peptide scaffolds. J Biomed Mater Res Part A 2017: 105A: 1856– 1866.
[2] Parvizi, M, Petersen, AH, van Spreuwel‐Goossens, CAFM, Kluijtmans, SGJM, Harmsen, MC. 2018. Perivascular scaffolds loaded with adipose tissue‐derived stromal cells attenuate development and progression of abdominal aortic aneurysm in rats. J Biomed Mater Res Part A 2018: 106A: 2494– 2506.

 

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